Review Article

Unwinding and Rewinding: Double Faces of Helicase?

Figure 2

Alignment of human DNA helicases that contain annealing activity. The annealing domain is indicated in yellow and the conserved helicase core domain in green; the conserved seven helicase motifs and the accessory domains are indicated. HP2, HepA-related protein domain 2; RBM, RPA-binding motif; HNH refers to the three conserved His and Asn amino acid residues in the motif. The total number of amino acids in each helicase protein is shown to the right. (a) It was reported that the C-terminus of BLM is important for its strand-annealing function [51, 53]; (b) it was also reported that the helicase core domain of PIF1 has annealing activity [66]. The functional annealing activity domain for CSB, TWINKLE, and Dna2 has not been mapped.
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