Straightforward Procedure for Laboratory Production of DNA Ladder

Figure 1

Schematic structure of pGEM-800 plasmid. The plasmid contains an insert of 800 bp which is a multimer of 100 bp repeats separated by the same unique SmaI restriction enzyme site (indicated by arrows). The GEM-1000F and GEM-1000R primers were designed to flank 800 bp insert plus 200 bp in length based on the pGEM sequence.