UvrD Participation in Nucleotide Excision Repair Is Required for the Recovery of DNA Synthesis following UV-Induced Damage in Escherichia coli
In the absence of UvrD, the nascent DNA at stalled replication forks is degraded in a manner similar to other repair mutants. [14C]-thymine-labeled cultures were pulse-labeled with [3H]-thymidine for 5 s before the cells were collected, resuspended in nonradioactive media, and UV-irradiated with 27 J/m2. The fraction of 14C-labeled genomic DNA (□) and 3H-labeled nascent DNA (■) remaining over time is plotted. Graphs represent the average of three independent experiments. The level of [3H] and [14C] in DNA immediately preceding irradiation ranged between 2500–7000 cpm and 1000–2500 cpm in all experiments. Error bars represent the standard error of the mean.
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