Practical Tips for Construction of Custom Peptide Libraries and Affinity Selection by Using Commercially Available Phage Display Cloning Systems
Figure 4
Unexpectedly isolated clones with high bias after library constructions. Bold “X” indicates any amino acids. (a) Stop codon appearance before the designated Cys. A combination of T7Select 415 vector and E. coli BL21 strain was used for in vitro packaging of the T7 phage, and individual clones were subjected to DNA sequencing. (b) Enrichment of a specific sequence and a mutation of the designated His (underlined). Randomly selected 12 individual clones of the M13 phage library were subjected to DNA sequencing. Parentheses indicate numbers of obtained clones.
