A Two-Piece Derivative of a Group I Intron RNA as a Platform for Designing Self-Assembling RNA Templates to Promote Peptide Ligation
Figure 1
Design of two-piece template RNAs derived from the Tetrahymena intron RNA. (a) Self-folding and self-assembling RNA templates for peptide ligation. Dashed lines indicate tertiary interactions that are crucial for 3D structure formation (left) and self-assembly (middle and right) of RNA templates. The substrate peptide-1 and -2 recognized by PRM-1 and -2 are indicated in red (peptide with C-terminal thioester) and green (peptide with N-terminal cysteine), respectively. (b) and (c) 2D (b) and 3D (c) illustrations of three-step construction of two-piece template RNAs from the Tetrahymena intron RNA. Step 1: the unimolecular Tetrahymena intron (Tet L-30 RNA) was divided into two pieces (P5 RNA and ΔP5 RNA). Step 2: the ΔP5 RNA was divided into two halves (Ez-5half and Ez-3half). Ez-5half is colored pale blue. Step 3: PRM-1 and-2 were added to P5- and Ez-5half, respectively.
