A Two-Piece Derivative of a Group I Intron RNA as a Platform for Designing Self-Assembling RNA Templates to Promote Peptide Ligation
Figure 2
A peptide ligation system with the two-piece Tetrahymena RNA template. (a) Secondary structures of the unimolecular Tetrahymena intron RNA (Tet L-30) and its two-piece derivatives. Boxes with dotted lines indicate P5/P5a elements at which the parent Tet L-30 was separated to produce a two-piece derivative (P5 : Ez). Dashed arrows indicate the tertiary interactions assembling the P5 and Ez RNA. Boxes with solid lines indicate peptide recognition modules (PRM1 and PRM2) introduced in the P5 RNA and Ez RNA, respectively. (b) Aminoacid sequences of substrate peptides employed for chemical ligation. Underlines indicate the sequences directly responsible for binding with their target RNAs.
