A Two-Piece Derivative of a Group I Intron RNA as a Platform for Designing Self-Assembling RNA Templates to Promote Peptide Ligation
Figure 4
Chemical ligation of six sets the substrate pairings in the presence of their cognate RNA templates. (a) 3D models of peptide recognition modules in substrate-template RNP complexes. RNA elements including PRM-1 and -2 are indicated in gray. Substrate peptides were indicated in red (λN-peptide), green (rev-peptide), and blue (P22N-peptide), respectively. (b and c) Product yields of 7-h chemical ligation (with 7.5 μM each peptide) in the presence of 10 mM Mg2+ ions. Reactions of the substrate peptides in the absence of template RNA are shown by white bars. Reactions in the presence of RNA templates having PRMs and lacking PRMs are shown by black and gray bars, respectively. (c) Product yields of 1-h chemical ligation (with 7.5 μM each peptide) in the presence of 10 mM Mg2+ ions. Reactions without RNA are shown by white bars. Reactions with RNA templates having PRMs and lacking PRMs are shown by black and gray bars, respectively.
