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Journal of Nucleic Acids
Volume 2012, Article ID 369058, 40 pages
http://dx.doi.org/10.1155/2012/369058
Review Article

Chemical Approaches for Structure and Function of RNA in Postgenomic Era

Departments of Pharmacology and Biochemistry, College of Medicine, Dong-A University, 3-1 Dong Dae Shin Dong, Seo Gu, Busan 602-714, Republic of Korea

Received 18 October 2010; Revised 21 June 2011; Accepted 5 July 2011

Academic Editor: Dmitry A. Stetsenko

Copyright © 2012 Tae Suk Ro-Choi and Yong Chun Choi. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

In the study of cellular RNA chemistry, a major thrust of research focused upon sequence determinations for decades. Structures of snRNAs (4.5S RNA I (Alu), U1, U2, U3, U4, U5, and U6) were determined at Baylor College of Medicine, Houston, Tex, in an earlier time of pregenomic era. They show novel modifications including base methylation, sugar methylation, 5 -cap structures (types 0–III) and sequence heterogeneity. This work offered an exciting problem of posttranscriptional modification and underwent numerous significant advances through technological revolutions during pregenomic, genomic, and postgenomic eras. Presently, snRNA research is making progresses involved in enzymology of snRNA modifications, molecular evolution, mechanism of spliceosome assembly, chemical mechanism of intron removal, high-order structure of snRNA in spliceosome, and pathology of splicing. These works are destined to reach final pathway of work “Function and Structure of Spliceosome” in addition to exciting new exploitation of other noncoding RNAs in all aspects of regulatory functions.