Chemical Approaches for Structure and Function of RNA in Postgenomic Era

Table 11

The analysis of [³²P]-labeled U2 RNA 5′ oligonucleotide [12]. The 5′ ends obtained from uniformly [³²P]-labeled U2 RNA were digested with T1 RNase or RNase A and isolated by two-dimensional electrophoresis (cellulose acetate at pH 3.5 followed by DEAE paper electrophoresis). The 5′ oligonucleotides were digested with snake venom phosphodiesterase before and after removal of 3′ phosphate with bacterial alkaline phosphatase. The products were separated as in Figure 27. The radioactivity ratios are listed.

5′ fragment from U2 RNA	^32P ratio in nucleotide
	Pi	pUm	pAm	pm32,2,7G	pC	pCp	pG	pGp
5′ oligo from T1 digestion	1.58	1.33	0.91	0.90	1			1.48
Alkaline phosphatase digested T15′ oligo	1.22	1.43	0.98	0.90	1		0.93
5′ oligo from RNase A digestion	1.74	1.25	1	1.08		1.50
Alkaline phosphatase digested A 5′ oligo	0.93	1.45	0.94	0.77	1