Review Article
Chemical Approaches for Structure and Function of RNA in Postgenomic Era
Table 9
The snRNA 2′ and 3′-OH labeling by NaIO4 oxidation and [3H]-KBH4 reduction [38]. The total 4–7S RNA from rat Novikoff hepatoma cell nuclei was labeled with [3H] by oxidation with NaIO4 followed by [3H]-KBH4 reduction (Figure 12). Individual RNA species were purified by gel electrophoresis (Figure 13). The RNA samples were hydrolyzed with 0.3 N KOH, and hydrolysates were chromatographed on whatman 3MM paper according to de Wachter and Fiers [55]. The radioactivities at the origin (22% for 5S RNA, 54.1% for U1 RNA, 49.7% for U2 RNA, and 50.6% for U3 RNA) represent % of total radioactivity applied and they represent the 5′ end labeling which was later elucidated by many enzymatic methods described in the text. The radioactivities moved by chromatography with standard nucleoside derivatives are the % of total in nucleosides derivatives. The A′ U′ G′ C′ represent trialcohol derivatives of nucleosides.
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