Structural and Functional Characterization of RecG Helicase under Dilute and Molecular Crowding Conditions

Figure 3

CD spectra of 1 μM DNA frayed duplex. Measurements were carried out at 37°C in 30 mM MES buffer (pH 7.0) containing 100 mM Na⁺, 0.5 mM Na₂EDTA, RecG (0 nM) and ATP (0 mM) (black), RecG (50 nM) and ATP (0.1 mM) (red), RecG (100 nM) and ATP (0.2 mM ) (green), RecG (150 nM) and ATP (0.3 mM, blue), RecG (200 nM) and ATP (0.4 mM, cyan), and RecG (250 nM) and ATP (0.5 mM) (pink), at (a) 0 wt% PEG 200 and (b) 40 wt% PEG 200.

(a)

(b)