Functional Annotation of Small Noncoding RNAs Target Genes Provides Evidence for a Deregulated Ubiquitin-Proteasome Pathway in Spinocerebellar Ataxia Type 1
ncRNA-mediated inhibition of HECTD1 causes more severe cytotoxicity in HEK293T cells. (a) Inhibition of HECTD1 by specific siRNA mixture increased significantly the lethality of cells transfected with the polyQ-expanded ATXN1 (ATXN182Q) vector as compared to wild-type ATXN1. (b) Inhibition of HECTD1 by miR-E1108 increased significantly the lethality of cells transfected with the polyQ-expanded ATXN1. Data are shown as mean values for clarity. Statistical analysis was performed using ANOVA. (c) Exogenous overexpression of polyQ-expanded ATXN1 leads to increased mRNA levels of HECTD1, RNF8, and PJA2.
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