Alternative Splicing of a Novel Inducible Exon Diversifies the CASK Guanylate Kinase Domain

Figure 7

E24a codes for a peptide insertion in the guanylate kinase domain of the CASK polypeptide. (a) Sequence of human guanylate kinase domain according to pdb structure, 1KGD, with secondary structure elements indicated as follows: arrows, beta strands; coils, alpha helices. Position of E24a-encoded peptide insertion is between residues Q835 and A836 (vertical arrow). (b) PyMOL representation of guanylate kinase domain from pdb structure, 1KGD [31]. Secondary structure elements are shown as in (a). The position of the E24a peptide insertion is indicated (vertical arrow). (c) The E24a peptide insertion was threaded onto the CASK guanylate kinase-like domain (1KGD) using MUSTER [32]. The E24a insertion is shown in pink.