Imaging mRNA Expression in Live Cells via PNA·DNA Strand Displacement-Activated Probes
Fluorescence activation of the strand-displacement probes by DNA. (a) Fluorescence spectra of the iNOS probe (0.2 M FAM-iNOS-PNA annealed to 0.4 M iNOS-DNA-DABCYL) following addition of 0.1, 0.2, 0.4, and 2 M iNOS DNA. Positive control: 0.2 M FAM-iNOS-PNA annealed to 0.4 M iNOS-DNA. (b) Fluorescence spectrum of the pLuc probe (0.2 M FAM-pLuc-PNA annealed to 0.4 M pLuc-DNA-DABCYL) following addition of 0.4 M iNOS-DNA. Positive control: 0.2 M pLuc-FAM-PNA annealed to 0.4 M pLuc-DNA. Excitation wavelength: 488 nm, temperature: 37°C.
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