Simultaneous Use of MutS and RecA for Suppression of Nonspecific Amplification during PCR

Figure 2

Various target sequences were amplified in the presence of ttRecA and ttMutS. (a) A 611 bp region of the ttha0122 gene was amplified from T. thermophilus HB8 genomic DNA template in the presence of ttRecA and ttMutS. The amounts of amplified fragments were quantified and are shown as bar graphs in the lower panel. Gray and blue bars indicate nonspecific and desired amplifications, respectively. (b) A 423 bp region of the ttha1806 gene was amplified from T. thermophilus HB8 genomic DNA template in the presence of ttRecA and ttMutS. (c) A 466 bp region of the ttha1300 gene was amplified from T. thermophilus HB8 genomic DNA in the presence of ttRecA or ttMutS. Note that relatively high concentrations of ttMutS were used here. (d) A 1,278 bp region of the ttha1548 gene was amplified in the presence of ttRecA or ttMutS. Note that relatively high concentrations of ttRecA were used here.