Multipyrene Tandem Probes for Point Mutations Detection in DNA
Figure 3
((a)–(d)) Fluorescent spectra of individual tandem components (⚫ = 3′-component, = 5′-component), their duplexes with fcDNA (◆ = 3′-component·fcDNA, ■ = 5′-component·fcDNA), and duplexes between tandems and fcDNA (★) for the following tandems: IVb+IIb (a), IIIb+Ib (b), IVa+IIa (c), IIIa+Ia (d). (e) Fluorescence intensity values at 480 nm for duplexes of individual tandem components (gray and black) with fcDNA in comparison with that of tandem duplexes with fcDNA (white). Conditions: 6.7 mM Tris-HCl (pH 8.9), 16 mM (NH4)2SO4, 3.6 mM MgCl2, nm, [conjugate] = [fcDNA] = M, °C.
