Multipyrene Tandem Probes for Point Mutations Detection in DNA
Figure 5
Differential melting curves of duplexes (IVb+IIb)·mmDNA (gray) and (IVb+IIb)·fcDNA (black) obtained upon excimer fluorescence detection. Melting curves were recorded with M solutions of duplexes in 6.7 mM Tris-HCl (pH 8.9), 16 mM (NH4)2SO4, and 3.6 mM MgCl2. Fluorescence values were registered at 480 nm at an excitation wavelength of 345 nm upon heating of samples at a rate of 0.7–1.5°C/min.
