Immobilization of DNA Aptamers on Polyester Cloth for Antigen Detection by Dot Blot Immunoenzymatic Assay (Aptablot)
Figure 1
Effect of oligonucleotide sequence. Different oligonucleotides (1, Apt 1; 2, Apt 2; 3, Apt 3; 4, Apt 4; 5, VT 1) were spotted on polyester cloth. The strips were either UV cross-linked (a) or not (b) and then saturated with thrombin solution followed by immunoenzymatic assay of bound thrombin as described in Methods. These experiments were repeated in a separate trial with identical results (not shown).
