In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element Specific for Bromacil

Table 3

Cross-reactivity data of R13.2 ssDNA MRE.

Eluent	Average fluorescence (RFU)	Standard deviation	value	Selectivity ratio
Bromacil	0.51	0.16	—	—
NT-1	0.22	0.20	0.06	2.3
NT-2	0.28	0.09	0.05	1.8
BSA	0.19	0.08	0.02	2.6
Atrazine	0.22	0.15	0.04	2.3
Malathion	0.10	0.15	0.02	5.2
0.01% MeOH	−0.0045	0.12	0.006	—

For each eluent, average fluorescence in solution is given with standard deviation. The value is given from a student’s -test performed between the eluent and bromacil. The selectivity ratio is the number of times greater binding to bromacil than to the eluent. All assays were performed in 0.01% methanol due to solubility; thus removal of R13.2 from bromacil was determined to be negligible.