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Journal of Nucleic Acids
Volume 2014, Article ID 475754, 7 pages
Research Article

Analysis of Nucleotide Sequences of the 16S rRNA Gene of Novel Escherichia coli Strains Isolated from Feces of Human and Bali Cattle

Department of Veterinary Public Health, Faculty of Veterinary Medicine, Udayana University, Jalan P.B. Sudirman, Denpasar, Bali 80232, Indonesia

Received 8 May 2014; Revised 30 July 2014; Accepted 30 July 2014; Published 9 September 2014

Academic Editor: Tatiana S. Oretskaya

Copyright © 2014 I Wayan Suardana. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Livestock especially cattle are known as a main reservoir of Escherichia coli O157:H7. This bacterium is considered as a pathogenic agent characterized by producing toxins, which are familiarly known as Shiga-like toxin-1 (Stx1) and Stx2. The aim of this work was to analyse the novel sequence of the 16S rRNA gene of strains isolated in this study in order to know the phylogenetic relationships between these sequences and those between the sequences of bacteria available in databanks. The results of this analysis showed that the strains KL-48(2) and SM25(1) that originated from human and cattle feces, respectively, are closely related among them and with respect to E. coli EDL 933, E. coli Sakai, E. coli ATCC 43894, E. coli O111:H-, E. coli O121:H19, E. coli O104:H4, and Shigella sonnei with more than 99% similarity values.