In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter

Figure 1

Illustration of the SELEX process. The SELEX process begins with 10¹⁵ ssDNA molecules and incubation with the target toxin B. Those that bind to toxin B are amplified and subsequently incubated with negative targets. Those that do not bind to negative targets are retained and amplified, thus completing one round of in vitro selection.