In Vitro Selection of a Single-Stranded DNA Molecular Recognition Element against Clostridium difficile Toxin B and Sensitive Detection in Human Fecal Matter

Figure 4

Sequence families of the round 12 library. Only the variable region is shown in the aligned subfamily of the CTAA superfamily. MRE sequences are aligned to the CTAA tetranucleotide sequence. Highlighted regions represent sequence homology shared in the subfamilies. represents the Gibbs free energy values. Possible structures indicate the number of predicted structures from the Mfold web server [33]. Percent homology is calculated from highlighted nucleotides divided by the length of the sequenced variable region.