Figure 4: Comparison of coefficient of determination () versus linker length for (a) direct labeling method and (b) indirect labeling method. Curves were fit to a one site specific binding with Hill slope model using GraphPad Prism. A (circle), C (square), G (triangle), and T (downward triangle) are the linkers used for IgE_17-4 aptamer. The remaining sequences (SM1, DM1_37, SA, and SM21) all possessed T linkers.