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Journal of Nucleic Acids
Volume 2017, Article ID 6067345, 8 pages
Research Article

The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding

Department of Molecular Genetics, Darmstadt University of Technology, Darmstadt, Germany

Correspondence should be addressed to H. Ulrich Göringer; ed.tdatsmrad-ut.oib@regnirog

Received 3 February 2017; Accepted 4 April 2017; Published 18 June 2017

Academic Editor: Luis A. Marky

Copyright © 2017 W.-Matthias Leeder et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure S1: SHAPE-reactivity profiles of the RPS12 pre-mRNA in dilute and crowded solvent conditions.

Supplementary Figure S2: Difference (dilute - crowded) SHAPE-reactivity plot of the RPS12 pre-mRNA in the absence of magnesium ions.

Supplementary Figure S3: Summary of the basepairing patterns of the RPS12-transcript at different solvent conditions.

Supplementary Table S1: Statistical comparison of the SHAPE-modification data.

  1. Supplementary Material