Putative HIV and SIV G-Quadruplex Sequences in Coding and Noncoding Regions Can Form G-Quadruplexes

Table 1

Melting temperatures and molecularities of the studied DNA oligonucleotides in the presence of 50 mM sodium and potassium ions.

		Potassium		Sodium
	Oligo.	Fold	[°C]	Fold	[°C]
	H2-U38	M, D	56.8	M, D	52.4
	H2-U22	M	56.6		ND
	H2-M15	M	57.1	M, D	46.6
	H1-JX	M	57.5	M > D	30.3,52.5
	H1-JX1	M	61.6	M > D	35.8, 51.8
	H1-L20	M	58.2	M	36.4, 50.4
	H2-U38B	M < D	59.5, 96.5	M	55.3
	H2-J0		51.8, 87.0		50.8
	H2-M15B	M	46.5		44.2
	S-M30	M	67.8	M	49.6
	S-JX	M, D	54.2, 79.5	M, D	35.0, 39.2
	H1-JN	M	48.5,46.5	M > D	38.7, 43.7
	H1-K02	M	48.4	M > D	32.1
	H1-M27	M	39.6	M	ND
	H1-K03	D, H	60.3, <98	M, D	39.7
	H1-JN-A	M	58.6		37.2

Smear represents flexible conformers; the mobility can correspond to unfolded structure; M: monomer; D: dimer; H: high ordered multimer; melting curve was obtained at ~264 nm; melting curve was obtained at ~293 nm. The error in determination is ±3°C.