Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage

<div>The effect of Fen-1 knockdown. (a) On repair of oxidative DNA damage. Top: western analysis of Fen-1 protein in AGS cells transfected with pLKO-1 sh Fen-1 to knock down Fen-1 or control vector; levels of PCNA protein were used as a loading control. Bottom: comet assay. AGS cells were treated with 20 <i>μ</i>M H<sub>2</sub>O<sub>2</sub> for indicated periods of time before being harvested for comet-Fpg/Endo III assay. (b) On DNA replication. S-phase cells were marked.</div>

Journal of Nucleic Acids

fig7

Figure 7

Figure 7: Overexpression of PCNA Attenuates Oxidative Stress-Caused Delay of Gap-Filling during Repair of UV-Induced DNA Damage 