Telomeric G-Quadruplexes: From Human to Tetrahymena Repeats

Figure 3

CD spectra of HTR (a and b) and THR (c and d) oligomers in a 25 mM mBR buffer (pH 7.0) in the presence of 50 mM KCl (red lines). The samples contain either PEG 200 or acetonitrile; 15% (v/w) (green lines), 30% (v/w) (light blue lines), and 50% (v/w) (dark blue lines). The increase in the magnitude of CD signals at 265 with increasing concentrations of dehydrating agent is marked by an arrow in both panels. Each DNA sample was annealed at 95°C for 5 min and then allowed to cool overnight at 4°C. The dashed red line represents the spectrum of THR without annealing.

(a)

(b)

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(d)