Selection of DNA Aptamers for Ovarian Cancer Biomarker CA125 Using One-Pot SELEX and High-Throughput Sequencing

Figure 1

In-tube ELISA tests in CA125-coated PCR tubes. Positive control (+) contains 30 μL of 8000 U/mL CA125. Negative control (−) was incubated with PBS overnight. Tube 1 was incubated with 30 μL of 8000 U/mL CA125 overnight at 4°C. Tube 2 contained 30 μL of 8000 U/mL CA125 and was dried without heating on a SpeedVac for 4 hr. Image taken with an iPhone 5s.