Selection of DNA Aptamers for Ovarian Cancer Biomarker CA125 Using One-Pot SELEX and High-Throughput Sequencing

Table 1

Characteristics of data resulting from Illumina sequencing. R0 is the unselected random DNA library; other numbers designate the SELEX round and whether the DNA was bound to CA125 (+) or was not bound (−).

SELEX round	DNA sequenced	Number of reads	Sequence duplication
R0	Library	7.3 × 10^6	15.24%
R1+	Bound	6.4 × 10^6	16.94%
R1−	Unbound	7.6 × 10^6	15.78%
R2+	Bound	5.7 × 10^6	45.24%
R2−	Unbound	10.8 × 10^6	18.00%
R3+	Bound	4.3 × 10^6	78.71%
R3−	Unbound	4.1 × 10^6	37.94%
R4+	Bound	7.4 × 10^6	85.04%
R4−	Unbound	5.4 × 10^6	82.62%