Effects of 5-Hydroxymethylcytosine Epigenetic Modification on the Stability and Molecular Recognition of VEGF i-Motif and G-Quadruplex Structures

Table 3

Comparison of and ΔH° of transition of iM containing 5-hmC ± KCl (100 mM) and ±40% acetonitrile (ACN) at pH 5.4. The unmodified control iM VEGF sequence () is also shown for comparison. Error measurements are omitted for clarity. Errors in ΔH° were less than 12% while errors in values were less than 3%.

	(°C)	ΔH° (kcal/mol)	Δ (°C)	ΔH° (kcal/mol)	(°C)	ΔH° (kcal/mol)	(°C)	ΔH° (kcal/mol)	Δ (°C)	ΔH° (kcal/mol)
Control	61	34	62	34	62	31	61	32	62	33
100 mM KCl Alone	62	27	61	29	61	41	61	35	61	28
40% ACN Alone	63	45	63	41	65	40	63	40	65	39
100 mM KCl + 40% ACN	59	36	62	32	62	35	57	44	62	35