Genetic Clearness Novel Strategy of Group I Bacillus Species Isolated from Fermented Food and Beverages by Using Fibrinolytic Enzyme Gene Encoding a Serine-Like Enzyme

Figure 3

Enzymatic activities of identified strains belonging to group I of Bacillus spp. BS: B. subtilis, Ba: B. amyloliquefaciens, Bl: B. licheniformis, Bp: B. pumilus, Bal: B. altitudinis, Bsa: B. safensis, Bat: B. atrophaeus. KE1 (pGEX-4T-1: fibEma-Ba /TOP10), KE2 (pGEX-4T-1: fibEma-Bs/TOP10), KE3 (pGEX-4T-1: fibEma-Bl/TOP10), KE4 (pGEX-4T-1: fibEma-Bp), KE6 (pGEX-4T-1: fibEma-Bsa/TOP10), KE7 (pGEX-4T-1: fibEma-Bal/TOP10), and KE8 (pGEX-4T-1: fibEma-Bat/TOP10). QEK1 (pGEX-4T-1: fibEma-Ba /BL21), QEK2 (pGEX-4T-1: fibEma-Bs/BL21), QEK3 (pGEX-4T-1: fibEma-Bl/BL21):, QEK4 (pGEX-4T-1: fibEma-Bp/BL21), QEK6 (pGEX-4T-1: fibEma-Bsa/BL21), QEK7 (pGEX-4T-1: fibEma-Bal/BL21), and QEK8 (pGEX-4T-1: fibEma-Bat/BL21).