Improved Methodology for Monitoring Poly(amidoamine) Dendrimers Surface Transformations and Product Quality by Ultra Performance Liquid Chromatography

Table 1

Comparison of HPLC and UPLC analyses of [EDA core] G = 4; NH₂ and G = 4; biotinylated PAMAM dendrimers. (^*Indicates dimer, ^†indicates unfunctionalized (G = 4; NH₂)).

Sample	Method	Retention time, tr (min)	WH/2 (min)	Theoretical Plates (N)
G = 4; NH2	HPLC	6.90	0.39	1737
		7.41^*	0.24	5290
	UPLC	4.08	0.10	9239
		4.21	0.07	20075
		4.27	0.07	20651
		4.37	0.07	21630
		4.44	0.09	13507
		4.81^*	0.26	1899
G = 4; biotinylated	HPLC	6.90^†	0.40	1651
		8.75	1.05	385
	UPLC	4.30^†	0.41	610
		4.98	0.28	1755
		5.48	0.14	8503
		5.80	0.13	11047
		6.11	0.20	5180
		6.30	0.14	11238
		6.47	0.17	8039