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Journal of Nanomaterials
Volume 2010, Article ID 906936, 9 pages
Research Article

Preparation and Characterization of Self-Assembled Nanoparticles of Hyaluronic Acid-Deoxycholic Acid Conjugates

College of Life Science, Ocean University of China, Yushan Road No. 5, Qingdao 266003, China

Received 14 October 2009; Accepted 31 March 2010

Academic Editor: Gaurav Mago

Copyright © 2010 Xuemeng Dong and Chenguang Liu. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Novel amphiphilic biopolymers were synthesized using hyaluronic acid (HA) as a hydrophilic segment and deoxycholic acid (DOCA) as a hydrophobic segment by a 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide mediated coupling reaction. The structural characteristics of the HA-DOCA conjugates were investigated using NMR. Self-assembled nanoparticles were prepared based on HA-DOCA conjugates, and its characteristics were investigated using dynamic laser light scattering, transmission electron microscopy (TEM), and fluorescence spectroscopy. The mean diameter was about 293.5 nm with unimodal size distribution in distilled water. The TEM images revealed that the shape of HA-DOCA self-aggregates was spherical. The critical aggregation concentration (CAC) was in the range of 0.025–0.056 mg/mL. The partition equilibrium constant () of pyrene in self-aggregates solution was from to . The aggregation number of DOCA groups per hydrophobic microdomain, estimated by the fluorescence quenching method using cetylpyridinium chloride, increased with increasing degree of substitution.