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Journal of Nanomaterials
Volume 2011 (2011), Article ID 105138, 8 pages
http://dx.doi.org/10.1155/2011/105138
Research Article

Single Nucleotide Polymorphism Detection Using Au-Decorated Single-Walled Carbon Nanotube Field Effect Transistors

1NanoBio Fusion Research Center, Korea Research Institute of Chemical Technology, Daejeon 305-343, Republic of Korea
2Regional Innovation Agency, Jeonbuk Technopark, Jeonju 561-844, Republic of Korea
3Panagene Inc., Daejeon 305-510, Republic of Korea
4Ucaretron Inc., Dongiltechno Building C, Anyang 431-716, Republic of Korea

Received 14 June 2010; Revised 3 September 2010; Accepted 10 September 2010

Academic Editor: Jianyu Huang

Copyright © 2011 Keum-Ju Lee et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

We demonstrate that Au-cluster-decorated single-walled carbon nanotubes (SWNTs) may be used to discriminate single nucleotide polymorphism (SNP). Nanoscale Au clusters were formed on the side walls of carbon nanotubes in a transistor geometry using electrochemical deposition. The effect of Au cluster decoration appeared as hole doping when electrical transport characteristics were examined. Thiolated single-stranded probe peptide nucleic acid (PNA) was successfully immobilized on Au clusters decorating single-walled carbon nanotube field-effect transistors (SWNT-FETs), resulting in a conductance decrease that could be explained by a decrease in Au work function upon adsorption of thiolated PNA. Although a target single-stranded DNA (ssDNA) with a single mismatch did not cause any change in electrical conductance, a clear decrease in conductance was observed with matched ssDNA, thereby showing the possibility of SNP (single nucleotide polymorphism) detection using Au-cluster-decorated SWNT-FETs. However, a power to discriminate SNP target is lost in high ionic environment. We can conclude that observed SNP discrimination in low ionic environment is due to the hampered binding of SNP target on nanoscale surfaces in low ionic conditions.