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Journal of Nanomaterials
Volume 2011, Article ID 404523, 6 pages
Research Article

Preparation and Characterization of Water-Soluble Chitosan Microparticles Loaded with Insulin Using the Polyelectrolyte Complexation Method

1Department of Pharmacy, Guangdong Food and Drug Vocational-Technical School, Guangzhou 510663, China
2College of Pharmacy, Guangdong Pharmaceutical University, Guangzhou 510006, China
3College of Public Health, Guangdong Pharmaceutical University, Guangzhou 510006, China

Received 26 July 2010; Revised 17 November 2010; Accepted 7 January 2011

Academic Editor: Maryam Tabrizian

Copyright © 2011 Sihui Wu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Polymeric delivery systems based on microparticles have emerged as a promising approach for peroral insulin delivery. The amount of insulin was quantified by the improved Bradford method. It was shown that water-soluble chitosan/insulin/tripolyphosphate (TPP) mass ratio played an important role in microparticles formation. Stable, uniform, and spherical water-soluble chitosan microparticles (WSC-MPs) with high insulin association efficiency were formed at or close to optimized WSC/insulin/TPP mass ratio. WSC-MPs had higher association efficiency in the pH 4.0 and pH 9.7 of TPP solution. The results showed that association efficiency and loading capacity of insulin-loaded WSC-MPs prepared in 0.01 mol/L HCl of insulin were 48.28 ± 0.90% and 9.52 ± 1.34%. The average size of insulin-loaded WSC-MPs was 292 nm. The presented WSC microparticulate system has promising properties towards the development of an oral delivery system for insulin.