Dynamics of Spreading and Alignment of Cells Cultured In Vitro on a Grooved Polymer Surface
Figure 5
Attachment of CHO cells. (a) Time-lapse phase contrast microcopy of a freshly harvested CHO cell on a grooved substrate. Scale bar, 10 m. (b) Orthogonal stack slices with time as the -axis (300 minutes, 150 slices). The cell elongates along the vertically oriented grooves and displays little lateral spreading. (c) Average minimum Feret's diameter of freshly harvested, floating CHO cells (grey bar) and CHO cells cultured for one day on a grooved substratum (open bar). Both evaluations were performed for n = 100 cells. (d) Time-lapse phase contrast microcopy of a freshly harvested CHO cells on a unpatterned smooth substrate.
