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Journal of Nanomaterials
Volume 2011, Article ID 423686, 8 pages
Research Article

Surface Modification of Titanium with Heparin-Chitosan Multilayers via Layer-by-Layer Self-Assembly Technique

1Department of Stomatology, Affiliated Hospital Academy of Military, Medical Sciences of Chinese PLA, Beijing 100071, China
2West China College of Stomatology, Sichuan University, Chengdu 610041, China
3Stomatology Department, Xuzhou Medical College, Xuzhou 221000, China
4College of Stomatology, Anhui Medical University, Hefei 230032, China

Received 4 June 2010; Revised 8 August 2010; Accepted 24 September 2010

Academic Editor: Xiaojun Yu

Copyright © 2011 Yao Shu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Extracellular matrix (ECM), like biomimetic surface modification of titanium implants, is a promising method for improving its biocompatibility. In this paper chitosan (Chi) and heparin (Hep) multilayer was coated on pure titanium using a layer-by-layer (LbL) self-assembly technique. The Hep-Chi multilayer growth was carried out by first depositing a single layer of positively charged poly-L-lysine (PLL) on the NaOH-treated titanium substrate (negatively charged surface), followed by alternate deposition of negatively charged Hep and positively charged Chi, and terminated by an outermost layer of Chi. The multilayer was characterized by DR-FTIR, SEM, and AFM, and osteoblasts were cocultured with the modified titanium and untreated titanium surfaces, respectively, to evaluate their cytocompatibility in vitro. The results confirmed that Hep-Chi multilayer was fabricated gradually on the titanium surface. The Hep-Chi multilayer-coated titanium improved the adhesion, proliferation and differentiation of osteoblasts. Thus, the approach described here may provide a basis for the preparation of modified titanium surfaces for use in dental or orthopedic implants.