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Journal of Nanomaterials
Volume 2011, Article ID 646538, 5 pages
http://dx.doi.org/10.1155/2011/646538
Research Article

Surface Modification of Gelatin Nanoparticles with Polyethylenimine as Gene Vector

Institute of Biomedical Engineering, National Taiwan University, Taipei 100, Taiwan

Received 29 August 2010; Revised 1 January 2011; Accepted 7 March 2011

Academic Editor: Rakesh Joshi

Copyright © 2011 Wei-Ti Kuo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A novel carrier on balancing the transfection efficiency and minimizing cytotoxicity was designed. Gelatin cross-linked with 1.8 kDa of PEI (GA-PEI 1.8 k) formed stable complex and resulted in high positive ζ potential (42.47 mV) and buffering effect. These nanoparticles with N/P ratio of 30 give high transfection efficiency 2 . 1 2 × 1 0 4  RLU/μg protein and cell viability (86.4%). These modified GA-PEI nanoparticles, with high transfection efficiency and low cell toxicity, can be a potential gene vector in gene therapy.