Nanobiosensor for Detection and Quantification of DNA Sequences in Degraded Mixed Meats

Figure 1

Schematic presentation of quantification and operating principles of swine nanobiosensor. Three oligonucleotides probes flanked by a hexyl-A spacer at both sides and alkanethiol cap at one-end and fluorophore at the other are shown to self-organize in a constrained arch-like structure where the fluorophore is quenched by the nanoparticle in the absence of any complementary targets. Upon target binding, the closed structure is opened into a rod-like conformation separating the fluorescent dye from the nanoparticle to allow fluorescence emission. The degree of fluorescence emission is shown to proportionate the degree of target hybridization, constituting the basis of quantitation. Gold nanoparticles and fluorophores are demonstrated by red and blue spheres. ssDNA and dsDNA are represented by single and cross-linked double lines.