Research Article

Nanobiosensor for Detection and Quantification of DNA Sequences in Degraded Mixed Meats

Figure 5

Pork detection in autoclaved pork-beef binary admixtures by porcine-specific nanobiosensor probes. From top to bottom are 100 : 0, 50 : 50, 25 : 75, 10 : 90, 5 : 95, 1 : 99, 0.5 : 99.5, 0.1 : 99.9, 1, 0 : 100, and 0 : 0 (free biosensor probes) pork-beef mixtures. The mixed and pure DNAs extracted from 2.5-h autoclaved meat mixtures were digested with AluI for 8 hrs, incubated with porcine-specific nanoconjugate probes for 60 min and finally the emission spectra were collected at 545 nm excitation. The final concentration of probes and targets were 10 nM and 60  g/mL. The LOD was found at 1% pork in autoclaved pork-beef binary admixture and is represented by the sky-blue curve (6th from the top).
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