Nanobiosensor for Detection and Quantification of DNA Sequences in Degraded Mixed Meats
Figure 6
Standard curves of the hybridization kinetics of swine nanobiosensor probes with synthetic (a) and AluI digested swine DNA extracted from raw-pork (b). The linear parts of the curves are represented by blue circles and also shown in the insets. The calculated and estimated probe/target ratios are shown on the left and right sides of each point (right curves). The probe/target ratios calculated from used synthetic targets are shown in the left curve. The final concentration of porcine nanoparticle probe was 10 nM in both cases with 9.01 × 1012 nanoparticles/mL. The copy number was calculated assuming 1.5 oligos/particle (50% particles bound to 2-oligo probes & 50% bound to 1-oligo probe). Each point was an average of three readings. The standard deviations at each point are shown in error bars. Note that quantitation is not valid at too low or too high concentration of target DNA.
