Fabrication of Biodegradable Polyester Nanocomposites by Electrospinning for Tissue Engineering

Figure 15

Immunofluorescent staining images of hSkMCs on the electrospun PCL/collagen nanofiber meshes: (a)–(c) randomly oriented and (e)–(g) aligned electrospun meshes; (a), (e) desmin-positive expression at 3 days after cell seeding, (b), (f) MHC-positive expression at 7 days after cell differentiation, and (c), (g) sarcomeric actin-positive expression at 7 days after cell differentiation. Laser confocal microscopy images of F-actin staining in hSkMCs seeded on the electrospun PCL/collagen nanofiber meshes (×600 magnification): (d) randomly oriented and (h) aligned electrospun meshes. Reproduced from Biomaterials with permission from Elsevier [21].