Dynamic Probing of Nanoparticle Stability In Vivo: A Liposomal Model Assessed Using In Situ Microdialysis and Optical Imaging

Figure 3

(a)The time-lapse optical imaging of liposome containing higher CF concentration at 100 mM illustrated no augmentation of fluorescence intensity in liver throughout the imaging course; the consistent self-quenching of fluorescence implicated the liposomes on site in liver remained their intact liposomal integrity. (B) At later phase of imaging course, the lysis buffer of Triton X-100 was locally infused at 2 𝜇l/min via microdialysis probe. It caused the rupture of liposomal lipid integrity, resulting in an immediate increase of CF fluorescence intensity measured in the microdialysate.