Design and Construction of a One-Dimensional DNA Track for an Artificial Molecular Motor

Figure 2

The 169 base-pair design template for a cassette L(ABC)₁R≡K₁ for building a motor track. The template contains an additional sequence, marked “internal primer,” which would allow for modification, for example, to attach a fiducial marker. Necessary design elements are noted: brackets mark repressor recognition sequences; arrows represent potential primer sites (also referred to as “handles” in the text); bars show where the template can be cut using restriction enzymes. In addition to the fully determinate bases (ATCG), the template also contains ambiguous bases (𝑁 for “any” base, 𝑆 for a strong base, i.e., C or G). This template describes a total of 2.60 × 10³³ distinct sequences.