Design and Construction of a One-Dimensional DNA Track for an Artificial Molecular Motor
Figure 6
(a) EcoRV digestion of the K8-lambda fragment ligation product shows that the ligation was successful, as evidenced by the extra high-molecular weight band in lane 3 compared with the digest of lambda DNA alone (lane 2). Lane 1 contains a 1 kb ladder with numbers on the left indicating fragment lengths in base pairs. (b) Biotinylation of the K8-lambda DNA construct, using short biotinylated duplex DNA, was successful. (A) EtBr-stained gel showing the expected molecular weight of the construct (open arrow). (B) Construct transferred to a nitrocellulose membrane, probed with streptavidin horseradish peroxidase, and visualized by chemiluminescence (ECL Plus, Pierce). Streptavidin binding coincides with the predicted size of the construct (filled arrow), indicating the presence of a biotin label on this high-molecular-weight DNA.
