Design and Construction of a One-Dimensional DNA Track for an Artificial Molecular Motor

Table 1

Sequence of the individual oligonucleotides used to construct the repetitive TW track; the top oligo of each pair is shown 5′ → 3′, while the complementary strand is shown in reverse 3′ → 5′ direction. Bold bases indicate the recognition sites for the TW protein feet. This primary cassette K₁ is shorter than 169 bp because it does not contain the sequence for the fiducial marker shown in Figure 2.

Handle L	GTATCATATGCTGAAGACGG; CATAGTATACGACTTCTGCCCACA
A	GTGTCATATAGCCAGAGACGTCTCGGCACGCGTC; GTATATCGGTCTCTGCAGAGCCGTGCGCAGCTCT
B	GAGAATTTAAATGTACTAGTTAACTAGTACGGTGA; TAAATTTACATGATCAATTGATCATGCCACTGAAG
C	CTTCAGCTCCTTAGGTTAACCTAACTCGAGCATA; TCGAGGAATCCAATTGGATTGAGCTCGTATCACA
Handle R	GTGTAGAGACCACTTAAGGCGTAA; TCTCTGGTGAATTCCGCATT