Nanopolymers Delivery of the Bone Morphogenetic Protein-4 Plasmid to Mesenchymal Stem Cells Promotes Articular Cartilage Repair In Vitro and In Vivo
Figure 2
Immunofluorescence staining for BMP-4 (a) and the mRNA levels (b) of chondrogenic markers. EGFP (green) and BMP-4 (red) were expressed in the experimental cells (MSCs transfected with BMP-4 plasmid by PLGA nanopolymers). The control cells (MSCs being transfected with naked BMP-4 plasmid alone) showed no BMP-4 expression (a). The mRNA expressions of Bmp4, Sox9, Col2a1, and Agg in the experimental cells were upregulated in comparison with those of the control cells (b). *, **.
