Basic Potential of Carbon Nanotubes in Tissue Engineering Applications
Figure 5
Effects of MWCNT on the expression of myogenic or adipose differentiation markers in C2C12 cells. C2C12 cells were exposed to MWCNTs (10 μg/mL) for 7 days, and RNA was isolated. mRNA levels for the downregulated (MyoD and Myogenin) and upregulated (PPARγ2, FABP4, Sox8, KLF15, and Nur-77) gene targets were determined by real-time PCR (a, b, and d). The mRNA levels were normalized using GAPDH as an internal control (mean ± SEM, , based on Student’s t-test). (c and e) Western blotting analysis after the exposure of C2C12 cells to MWCNT. β-Actin was used as a loading control [47].