Review Article

Basic Potential of Carbon Nanotubes in Tissue Engineering Applications

Figure 5

Effects of MWCNT on the expression of myogenic or adipose differentiation markers in C2C12 cells. C2C12 cells were exposed to MWCNTs (10 μg/mL) for 7 days, and RNA was isolated. mRNA levels for the downregulated (MyoD and Myogenin) and upregulated (PPARγ2, FABP4, Sox8, KLF15, and Nur-77) gene targets were determined by real-time PCR (a, b, and d). The mRNA levels were normalized using GAPDH as an internal control (mean ± SEM, 𝑛 = 3 , 𝑃 < 0 . 0 1 based on Student’s t-test). (c and e) Western blotting analysis after the exposure of C2C12 cells to MWCNT. β-Actin was used as a loading control [47].
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