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Journal of Nanomaterials
Volume 2012, Article ID 454507, 7 pages
http://dx.doi.org/10.1155/2012/454507
Research Article

Fabrication of Photofunctional Nanoporous Membrane and Its Photoinactivation Effect of Vesicular Stomatitis Virus

1Department of Chemistry, Yonsei University, Shinchon-Dong 134, Seodaemun-Gu, Seoul 120 749, Republic of Korea
2Department of Microbiology, College of Medicine, Yonsei University, Seoul 120 752, Republic of Korea
3Cardiology Division, Cardiovascular Center, College of Medicine, Yonsei University, Seoul 120 752, Republic of Korea

Received 29 May 2011; Accepted 11 July 2011

Academic Editor: Amir Kajbafvala

Copyright © 2012 Kang-Kyun Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Fabrication and photophysical study of photofunctional nanoporous alumina membrane (PNAM) were performed, and its application of photodynamic antimicrobial chemotherapy (PACT) was investigated. Nanoporous alumina membrane (NAM) was fabricated by two-step aluminium anodic oxidation process. Surface of the fabricated NAM was modified with organo-silane agent to induce covalent bonding between NAM and a photosensitizer (PtCP: [5,10,15-triphenyl-20-(4-methoxycarbonylphenyl)-porphyrin] platinum). PtCP was covalently bonded to the surface of the modified NAM by nucleophilic acyl substitution reaction process. The morphology and the photophysical properties of the fabricated PNAM were confirmed with field emission scanning electron microscope (FE-SEM), steady-state spectroscopies, and nanosecond laser-induced time-resolved spectroscopy. For the efficacy study of PNAM in PACT, an enveloped animal virus, vesicular stomatitis virus (VSV), was utilized as a target organism. Antiviral effect of the PNAM-PACT was measured by the extent of suppression of plaque-forming units (PFU) after the light irradiation. In the cultures inoculated with PACT-treated VSV, the suppression of PFU was prominent, which demonstrates that PNAM is a potential bio clean-up tool.