Single-Molecule Sandwich Immunoassay for Quantification of Alpha-Fetoprotein Based on Evanescent Field-Enhanced Fluorescence Imaging
Figure 3
(a) Peak of fluorescence intensity of serially diluted AFP standard antigen reacted with a chip as “a” to “b” horizontal line in (b). (i) 14.3 aM; (ii) 7.2 fM; (iii) 14.3 fM; (iv) 7.2 pM; (v) 14.3 pM; (vi) 143 pM. RFI: relative fluorescence intensity.
